Saturday, October 24^th: 1-4 pm

Presented by Dr. Vincenzo Venditti (Professor, Chemistry Dept., ISU) and Dr. Christopher G. Williams (Biomolecular NMR Facility Manager, ISU)

1:00 – 2:00 pm : Principles and applications of solvent paramagnetic relaxation enhancement

 Lecture hall MBB 1414

Dr. Venditti will introduce the physical basis of solvent paramagnetic relaxation enhancement (sPRE) and will discuss how residue-resolved sPRE measurements can be used to characterize conformational heterogeneity, map surface potentials, and constrain models of biomolecular interactions. The lecture will also cover experimental design, appropriate controls, and strategies for interpreting sPRE profiles in the context of protein structures and conformational ensembles.

2:00 – 4:00 pm : Practical demonstration: data acquisition, processing, and analysis

  Computational lab MBB 1340

Participants will see how paramagnetic and reference measurements are set up and acquired, how the resulting spectra are processed, and how residue-specific PRE values are extracted. The session will also demonstrate how experimental sPRE profiles can be mapped onto protein structures and compared with candidate conformations or structural ensembles.