Workshop : 15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics

Saturday, October 18th: 1-4 pm

Presented by Dr. Vincenzo Venditti and Dr. Jeffrey Purslow

1:00 – 2:00 pm : Introduction to CPMG Relaxation Dispersion (Lecture hall MBB 1414)

Dr. Venditti will provide an overview of the theory and practical applications of 15N Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments in solution NMR. The session will cover how these experiments can be used to characterize conformational exchange processes in proteins on the microsecond-to-millisecond timescale, including data interpretation and fitting strategies.

2:00 – 4:00 pm : Hands-on session (Computational lab MBB 1340)

Led by Dr. Purslow, this interactive session will guide participants through data analysis workflows using example datasets. Attendees will gain practical experience in processing and fitting relaxation dispersion profiles using common software tools. One-on-one consultations will be available to help troubleshoot individual datasets or explore specific use cases. Participants will receive a curated set of pulse programs, data processing scripts, and analysis tools to take home for use in their own labs.

Attendees are encouraged to bring their own data and laptops for personalized guidance.

For background reading and a detailed protocol, see the open-access JoVE article: https://www.jove.com/v/62395