Workshop : 15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics

Saturday, October 18th: 2:30-5:30 pm

2:30 – 3:15 PM – Introduction to CPMG Relaxation Dispersion

Speaker: Dr. Venditti
Dr. Venditti will provide an overview of the theory and practical applications of Carr–Purcell–Meiboom–Gill (CPMG) relaxation dispersion experiments. The session will discuss how these experiments are used to characterize conformational exchange processes in proteins on the microsecond-to-millisecond timescale, including strategies for data interpretation and model fitting.

3:15 – 3:30 PM – Break

3:30 – 5:30 PM – Hands-on Session

Instructor: Dr. Purslow
This interactive session will guide participants through the processing and fitting of relaxation dispersion profiles using a dataset collected in Dr. Venditti’s lab.

• – The detailed protocol is available as an open-access JoVE article: https://www.jove.com/v/62395

      – Raw spectra and scripts are available here: https://drive.google.com/drive/folders/1QfUfDFosXAGgAwN7eaTVJ9VzJVPzweg6?usp=drive_link

One-on-one consultations will be available to troubleshoot individual datasets or explore specific applications. Participants will also receive a curated set of pulse programs, processing scripts, and analysis tools to take home for use in their own labs.

Please bring your own laptop and data if you’d like personalized guidance. A limited number of computers with Sparky and MATLAB installed will be available for use during the workshop.